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. 2021 Jan 14;11:609600. doi: 10.3389/fpls.2020.609600

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Copyright © 2021 Saccomanno, Potocký, Pejchar, Hála, Shikata, Schwechheimer and Žárský.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphorylation of EXO70C2 regulates its inhibitory function in tobacco pollen tubes. Quantitative analysis of growth rate (A) and thickness (B) in tobacco pollen transiently expressing 4 μg of YFP:AtEXO70C2 wild type (WT), phospho-dead (PD) and phospho-mimetic (PM) variants. Pollen tubes expressing YFP:GUS and Arabidopsis YFP:EXO70A2 were used for comparison. Measurements were performed on 40 or more transformed pollen tubes per variant imaged during three independent experiments. Different letters indicate significant differences between samples at P < 0.05.