FIGURE 5.

Expression of phospho-dead EXO70C2 differently affects the growth of Arabidopsis wild type and exo70C2 mutant pollen tubes. Quantitative analysis of pollen tube length of Arabidopsis wild type (WT) (A) and exo70C2 mutant (B) expressing EXO70C2p:AtEXO70C2:GFP wild type, phospho-dead (PD), and phospho-mimetic (PM) variants. Pollen tubes from Col-0 ecotype and exo70C2 mutant that did not express any GFP-fused AtEXO70C2 variant were used for comparison as positive and negative controls, respectively. Measurements were performed on 148 or more pollen tubes for each variant imaged during three independent experiments. Different letters indicate significant differences between samples at P < 0.05.