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. 2021 Jan 14;11:582998. doi: 10.3389/fimmu.2020.582998

Figure 1.

Figure 1

Hemizygous deletion of Arg1 in myeloid cells of APP Tg2576 mice promotes Aβ deposition. We performed immunohistochemistry staining for species of Aβ (total Aβ, Aβ 42, Aβ 40, Aβ 38) and Congophilic Aβ deposition in the anterior cortex (ACX), hippocampus (HPC), and entorhinal cortex (ECX) of 15-month old APP Tg2576 mice from two groups (APP+/−/Arg1+/+/LysMcreTg/+, APP+/−/Arg1fl/+/LysMcreTg/+). (A–J) Representative images for total Aβ (A, B), Aβ 42 (C, D), Aβ 40 (E, F), Aβ 38 (G, H), and Congo red (I, J) are presented. (K–Q) Quantification analysis of total Aβ (K), Aβ 42 (L), Aβ 40 (M), Aβ 38 (N), and Congo red (O) are shown. ELISA was performed for Aβ 42 and Aβ 40 in the cortex. (P, Q) Quantification analysis of Aβ 42 (P) and Aβ 40 (Q) by ELISA for both detergent soluble fraction and formic acid fraction. Quantification of Aβ species and Congo red from staining was determined using six sections per mouse. For mice in each group, n = 6–7 for APP+/−/Arg1+/+/LysMcreTg/+, n = 6–9 for APP+/−/Arg1fl/+/LysMcreTg/+. *p < 0.05; **p < 0.01. Unpaired Student’s t-test. Values represent mean ± SEM. Scale bars represent 500 µm for images and 20 µm for insets.