Fig. 2.

(A) Peptide array for serum from sheep experimentally infected with Fasciola hepatica (N = 4) at four and nine weeks post infection (wpi), and cattle experimentally infected with F. hepatica (N = 3) at four and 11 wpi. (B) Sheep (N = 3), calves at the September time point (N = 5) and adult cattle (N = 8) naturally infected with F. hepatica. Recombinant cathepsin L1 (rCL1) protein sequence is divided in peptides of 15 amino acids in length (see materials and methods). Separate arrays are indicated by the horizontal black line. Control anti-sheep and anti-bovine samples were included as shown for individual array. Intensity of colour is used to show of host antibody recognition, (represented by mean pixel density), white meaning no antibody binding, through to red which shows strong binding. Regions 1, 2, 3 and 4 with the strongest antibody recognition by experimentally infected animals are highlighted. Control protein spots of whole excretory/secretory (ES) products, rCL1 and the tertiary antibody (anti-mouse IgG) are as shown for each serum sample. For experimentally infected sheep and first naturally infected bovine array, no data is available for ES antigen and rCL1 protein spots.