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. 2021 Jan;148:105189. doi: 10.1016/j.nbd.2020.105189

Fig. 7.

Fig. 7

Decreased VGNC expression and increased PKC and ERK activity in Tm4sf2−/y mice. A) Western blot of crude membrane preparation from Tm4sf2+/y and Tm4sf2−/y mice habenulae detected using ChemiDoc XRS+ System (BioRad) and quantification of voltage-gated sodium and potassium channel protein levels normalized on tubulin. You can see a downward trend and a significant reduced expression for NaV1.1 and NaV1.6 respectively in Tm4sf2−/y mice, while no changes for Kv4.2 has been detected; B) Quantification of PKC brain activity obtained using a PKC Kinase Activity Assay Kit showing a hyperactivity in Tm4sf2−/y compared to Tm4sf2+/y mice; C) Western blot of habenulae homogenates from Tm4sf2+/y and Tm4sf2−/y mice and quantification of expression levels represented as pERK/ERK ratio. α-tubulin was used as loading control.