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. 2021 Jan 14;11:609689. doi: 10.3389/fimmu.2020.609689

Figure 5.

Figure 5

CG-598 enhances barrier function and immune regulation during colitis. Mice were fed with 2.5% DSS in drinking water for five days and followed by normal water through the end of the experiments (n=9 per group). Mice were treated with 15 mg/kg CG-598 by oral gavage once every day. Colon tissues were analyzed at day 11. (A) E-cadherin staining (×400 magnification). (B) Relative mRNA expression of E-cadherin and (C) itf to gapdh. (D) Periodic acid–Schiff staining of colon tissues. (×200 magnification) (E) Relative mRNA expression of muc2 and muc3 and (F) IL-22 and IL-10 in colon tissue. (G, H) CD4+ T-cells or CD8+ T-cells were sorted from lamina propria of colon tissues at day 11 and stimulated with anti-CD3/CD28 antibodies overnight. The levels of IL-22 from T-cells and IL-10 from CD4+ T-cells were analyzed from culture supernatants. One-way ANOVA. Data are shown as mean ± SEM, ns, not significant, *p < 0.05, **p < ***p < 0.001.