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. 2021 Jan 14;10:608298. doi: 10.3389/fcimb.2020.608298

Figure 1.

Figure 1

The 33 RNA-Seq data sets used for expression analysis. (A) The time points indicate when RNA-Seq samples were collected and the associated C. parvum life cycle stage. The schematic model of the C. parvum life cycle is modified from (Tandel et al., 2019). “Culture block” refers to the developmental stage where parasites in traditional in vitro cultures fail to progress. (B) Principal component analysis of 33 C. parvum transcriptomes. The analysis is based on the normalized expression level (VST) of the C. parvum mRNA and predicted lncRNA genes. Samples collected from different time points are indicated by colors. Various projects/batches are represented by shapes. Batch A includes sample IDs 3–19 without host cells. Batch B includes sample IDs 1, 23–26 from IPEC host cells. Batch C includes sample IDs 27–30 from MDBK host cells. Batch D contains sample IDs 2, 20–22, 31–33 from HCT-8 host cells (Table 1).