Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 14;11:531385. doi: 10.3389/fpsyt.2020.531385

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Konturek, Martinez, Niesler, van der Voort, Mönnikes, Stengel and Goebel-Stengel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Western blot for BDNF (1:5,000) in IBS patients and control subjects. Lane 1 contains the molecular weight standards. Lane 2 contains colonic wall protein obtained by deep tissue biopsy from mucosa/submucosa in patients with IBS (14 female and six male, pooled sample) and lane 3 colonic wall protein from control subjects (two female and eight male, pooled sample, A). Same blot after washout of ponceau staining solution and application of primary anti-BDNF antibody (1:5,000) and secondary antibody goat anti-rabbit AP (1:2,000). Lane 1 contains molecular standards highlighted with pen. Lane 2 and 3 show detection of multiple bands with similar intensity for patients with IBS and healthy controls, respectively. For BDNF (UniProt P23560) multiple Western Blot bands are possible and expected. The strongest band was found between 49 and 62 kDa most likely representing glycosylated prepro-/pro BDNF dimer. Weaker bands were detected at ~ 45, 38, and ~ 30 kDa likely related to prepro-/pro BDNF (B).