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. 2021 Jan 14;7:613158. doi: 10.3389/fnut.2020.613158

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Copyright © 2021 Chaklader, Howieson, Fotedar and Siddik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serum biochemical changes (A–G) in pre-challenge and 24-h post-challenge juvenile barramundi fed control, 60% FM replacement diet (60PBM + HI), and 75% FM replacement diet (75PBM + HI), supplemented with 10% full-fat HI larvae. The bar represents the mean of three technical replicates. The violet and the red markers (each marker represents the mean of two biological replicates) denote pre-challenge and post-challenge groups, respectively. P-values on the top of the bar with scatter dot plot denote significant differences between pre- and post-challenge groups fed control and HI-supplemented PBM diets (paired t-test, P < 0.05). The effect of diet and challenge and their interaction were analyzed by two-way ANOVA with Dunnett's multiple-comparisons test.