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. 2017 Jan 26;25(2):177–186. doi: 10.3727/096504016X14732772150343

Figure 5.

Figure 5

(A) Wound-healing assay and (B) Transwell assay were used to determine the migration and invasion of U2OS cells treated with recombinant human CXCL5. Nontreated U2OS cells were used as the control group. **p < 0.01 versus Control. (C) Western blot and (D) ELISA were conducted to examine the protein expression and secreted protein levels of CXCL5 in hFoB1.19 cells transfected with CXCL5 expression plasmid or blank vector as control. **p < 0.01 versus Control. We further used the CM of these hFoB1.19 cells to culture U2OS cells. (E) Wound-healing assay and (F) Transwell assay were used to determine the migration and invasion of U2OS cells. **p < 0.01 versus Control.