Figure 1.

DPR1 is required for LPS-induced mitochondrial fragmentation. (A) Representative immunoblot analysis and quantification of total DRP1 protein in NT-Control and DRP1 KD immortalized Bone marrow- derived macrophage cell lines which were left untreated (Mock) or stimulated for 4h with 200 ng/ml LPS. (B) Representative confocal immunofluorescence images of NT-control and DRP1 KD macrophages stained for TOM20 (red), Complex I (green) and DAPI (blue) after 4h LPS stimulation. (C) Quantification of mitochondrial length and fragmentation was performed based on Complex I images of Mock and LPS-stimulated macrophages using CellProfiler image analysis software. Graphs are presented as mean of n=4 independent experiments with standard deviation (SD) error bars. P value was calculated using two-way ANOVA with Sidak’s post-test for multiple comparisons. *P < 0.05; **P < 0.01; ***P < 0.001.