Figure 1.

CASC9 enhances the glycolysis of pancreatic cancer cells. A. The relative expression of CASC9 was compared between different pancreatic cancer cell lines (BxPC-3, MiaPaCa-2, PANC-1, and SW1990) and a normal pancreatic duct cell line (HPDE6-C7) by using qRT-PCR. SW1990 and PANC-1 cell lines were transfected with Si-CASC9 or Si-NC. B. The relative mRNA level of CASC9 was detected by qRT-PCR. C. The ability of relative glucose uptake was measured by the glucose uptake assay. D. The relative production of lactate in each culture medium was determined by the lactate release assay. E. Western blot analysis was used to detect the protein levels of the key glycolytic enzymes HK2, GLUT4, and LDHA. Then, two pancreatic cancer cell lines were transfected with the pcDNA-CASC9 or pcDNA-NC plasmid. F. The mRNA level of CASC9 was determined by qRT-PCR. G and H. The relative abilities of glucose uptake and lactate production were measured by glucose uptake and lactate release assays, respectively. I. The protein levels of HK2, GLUT4, and LDHA were detected by western blot analysis. The results shown are from three independent experiments. The mRNA value was normalized to GAPDH. The lactate level was normalized to total protein of each sample. *, P < 0.05; **, P < 0.01; ***, P < 0.001.