Figure 2.

CASC9 enhances the EMT phenotype of pancreatic cancer cells. SW1990 and PANC-1 cells were transfected with Si-CASC9 or Si-NC and then subjected to the Transwell migration and invasion assays. A. The relative protein levels of Snail, Vimentin, N-Cadherin, and E-Cadherin were measured by western blot analysis. B. Representative images of migrated and invaded cells are shown on the membrane of the lower chamber. C. The relative abilities of migration and invasion are presented by quantification of stained cells. D. Two cell lines were transfected with the pcDNA-CASC9 or pcDNA-NC plasmid, and western blot analysis was used to detect the expression of Snail, Vimentin, N-Cadherin, and E-Cadherin. E and F. After transfection with pcDNA-CASC9 or pcDNA-NC, the relative migratory and invasive abilities in the two cell lines were determined by Transwell migration and invasion assays, respectively. The graphs presented are from three independent assays. Scale bar, 100 μm. **, P < 0.01.