Figure 4.

HIF-1α activation partially mediates CASC9-induced glycolysis and EMT phenotype. The two pancreatic cancer cell lines were cotransfected with pcDNA-CASC9/pcDNA-NC and Si-HIF-1α/Si-NC. A. The protein levels of HK2, GLUT4, LDHA, Snail, Vimentin, N-Cadherin, E-Cadherin, and HIF-1α were detected by western blot analysis. B and C. The relative abilities of glucose uptake and lactate production were determined by the glucose uptake and lactate release assays, respectively. D-F. The Transwell migration and invasion assays were used to determine the relative migratory and invasive abilities of the two cell lines. The data shown are the results of three independent experiments. The lactate level was normalized to total protein of each sample. Scale bar, 100 μm. *, P < 0.05; **, P < 0.01.