Figure 5.

AKT activation participates in the enhancement of glycolytic flux and EMT partially mediated by CASC9-induced HIF-1α activation. SW1990 and PANC-1 cells were transfected with pcDNA-CASC9 or pcDNA-NC, followed by treatment with 20 μM LY294002 for 24 h. A. Western blot analysis was used to detect the protein levels of HK2, GLUT4, LDHA, Snail, Vimentin, N-Cadherin, E-Cadherin, HIF-1α, and p-AKT. B and C. The relative abilities of glucose uptake and lactate release were determined by the glucose uptake and lactate release assays, respectively. D-F. The relative migratory and invasive abilities of the two cell lines were measured by the Transwell migration and invasion assays, respectively. The graphs shown are from three independent assays. The lactate value was normalized to total protein of each sample. Scale bar, 100 μm. *, P < 0.05; **, P < 0.01.