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. 2021 Jan 1;11(1):138–156.

Figure 4.

Figure 4

Inhibition of miR-125a-5p reverses the silencing effect of circPARP4 in glioma cells. A. The expression of circPARP4 and miR-125a-5p was determined in U87 and U251 cells transfected with miR-125a-5p inhibitor or control. B. The proliferation ability of glioma cells in different conditions of miR-125a-5p and circPARP4 expression pattern was assessed by CCK-8. C. The colony number of glioma cells with different treatments was measured via colony formation assays and quantified at 72 h. D. The effects of miR-125a-5p inhibition and knocking down circPARP4 on glioma cell apoptosis were analyzed by flow cytometry and quantified at 72 h. E. Cell migration and invasion of U87 and U251 in different conditions were assessed by transwell assays. F. The expression of EMT markers affected by miR-125a-5p and circPARP4 was determined by western blot. Data indicate the mean ± SD, n = 3. **P<0.01, ***P<0.001 vs. control and ##P<0.01, ###P<0.001 vs. si-circ.