Fig. 6. CoRest-C, Rpd3, and CBP colocalize to the specific gene loci.

a Schematic diagram of the preparation of MB nuclei. The KASH domain, which is inserted to the outer membrane of the nuclear envelope, was tagged with FLAG, and expressed in MB neurons using the MBp-LexA driver⁴³. The FLAG-labeled MB nuclei were collected by immunoprecipitation with the anti-FLAG antibody. b ChIP-seq signals at the region near kay. FLAG-KASH and CsChrimson were expressed by MBpLexA. CoRest-C::myc was expressed by MBsw. MB nuclei prepared from naïve flies or flies illuminated with red light were subjected to ChIP-seq analysis, using anti-myc (n = 3), anti-CBP (n = 3), or anti-Rpd3 (n = 3) antibodies. The sequencing data of input DNA were shown at the bottom. Overlapped binding sites determined by peak calling are highlighted by a yellow vertical bar. The y axes show the number of mapped reads, and the upper limits were adjusted to the number of total reads in each sample. c–e Aggregate gene plot. The mapped reads of each sample were summarized with respect to the distance from the transcriptional start site (TSS) and the transcriptional end site (TES). f Venn diagram showing overlaps in CoRest-C-, CBP-, and Rpd3-binding sites. g Heatmap of the log2 fold change in expression of 2702 genes significantly altered after 5 min of optogenetic activation followed by 10 min of rest. Increased genes, 1582; decreased genes, 1120. h Venn diagram showing the overlap in CoRest-C-, CBP-, and Rpd3-binding sites in the 1582 genes showing increased expression in g.