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. 2017 Jan 26;25(2):241–248. doi: 10.3727/096504016X14742891049118

Figure 4.

Figure 4

Downregulation of Rab23 inhibits the activity of the Shh/Gli1 signaling pathway. (A) The protein expression levels of Shh and Gli1 were remarkably decreased in DU145 cells by downregulation of Rab23, compared to the control group. (B–D) DU145 cells were transfected with Rab23 siRNA or control siRNA in the presence or absence of the Gli1 inhibitor GANT-61 (100 nM) for 24 h. The MTT assay was performed to measure cell proliferation. The Transwell assay was performed to measure cell migration and invasion. The results showed that GANT-61 significantly potentiated the inhibitory effect of Rab23 siRNA on the proliferation, migration, and invasion of DU145 cells. *p < 0.05.