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. 2017 Jan 2;25(1):43–53. doi: 10.3727/096504016X14719078133285

Figure 5.

Figure 5

miR-17-5p directly targets 3′-UTR of beclin-1. (A) The predicted binding sequences for beclin-1 3′-UTR with miR-17-5p. (B) The interaction between miR-17-5p and beclin-1 was determined by luciferase activity assays. The wild-type or mutant beclin-1 3′-UTR was cotransfected with miR-17-5p mimics or scramble miRNA and incubated for 48 h into U-87 cells followed with a detection by dual-luciferase reporter assay kit. WT denotes pGL3 vectors containing beclin-1 3′-UTR; MUT denotes pGL3 vectors containing mutated beclin-1 3′-UTR. **p < 0.01 compared with the indicated group.