Figure 6.

Exotic expression of miR-17-5p decreased autophagy activity via targeting beclin-1. (A) U87 cells were transfected with miR-17-5p mimics with or without beclin-1 overexpression plasmids. Then the cells were subjected to irradiation, and the autophagy activity was tested by Western blot. (B–E) Relative levels of ratio of LC3-II/I (B), p62 (C), beclin-1 (D), and Atg7 (E) were measured using ImageJ and normalized to GAPDH. Data are presented as mean ± SD from at least three independent experiments. **p < 0.01 compared with control group and ##p < 0.01 compared with the indicated groups.