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Depletion of NFBD1 activates the mitochondrial apoptosis pathway. (a) Measurement of mitochondrial membrane potential (MMP) using JC-1 by flow cytometry (FCM). (b) The expression of apoptotic factors, including the cleavage of caspase 3, caspase 9, and PARP. (c) Changes in mitochondrial and cytosolic cytochrome c levels. (d) Expression of apoptosis-related proteins.
