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. 2021 Jan 27;12:608. doi: 10.1038/s41467-020-20801-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Workflow: analysis of Ntn1^flox/flox and CAGGS:Cre^ERT2; Ntn1^flox/flox mice 8 weeks after Cre induction for (b–e). b Representative flow cytometry plots of the LSK population of Ntn1^flox/flox and Ntn1^{ΔCAGGS/ΔCAGGS} mice. c Frequencies of bone marrow HSCs in Ntn1^flox/flox and Ntn1^{ΔCAGGS/ΔCAGGS} mice; n = 7 (flox)–10 (∆CAGGS), two independent experiments. d Cell cycle phase of HSCs derived from Ntn1^flox/flox and Ntn1^{ΔCAGGS/ΔCAGGS} mice; n = 8 (flox) and 10 (∆CAGGS), two independent experiments. e Relative expression of quiescence and activation related genes in HSCs derived from Ntn1^flox/flox and Ntn1^{ΔCAGGS/ΔCAGGS} mice; n = 6 (flox)–9 (∆CAGGS), two independent experiments. f Frequencies of bone marrow HSCs in Ntn1^flox/flox and Ntn1^{ΔCAGGS/ΔCAGGS} mice 5 months after Cre induction; n = 8 (∆CAGGS) and 12 (flox), three independent experiments. g Cell cycle phase of HSCs derived from Ntn1^{+/LSL-Rosa26-Ntn1} and Ntn1-OE mice; n = 8 (∆CAGGS) and 12 (flox), three independent experiments. h Workflow: competitive transplantation of CAGGS:Cre^ERT2, Ntn1^{ΔCAGGS/ΔCAGGS} and Ntn1-OE mice 5 months after Cre induction, analysed in (i–k). i Representative FACS plots of peripheral blood leucocytes pre-gated on CD45⁺ cells at 16 weeks after transplantation. j Peripheral blood CD45.2⁺ chimerism during competitive transplantations; n = 13 (OE)–14 (Cre/∆CAGGS), two independent experiments. Analysis was done with two-way-ANOVA, multiple comparison with LSD Fisher’s test. k Bone marrow HSC CD45.2⁺ chimerism after 16 weeks of competitive transplantation; n = 12 (Cre)–13 (∆CAGGS/OE), two independent experiments. l Workflow: transplantation of 200 HSCs sorted from CAGGS:Cre^ERT2 and Ntn1^{ΔCAGGS/ΔCAGGS} mice at 5 months after Cre induction. m Frequencies of bone marrow HSCs 8 weeks transplantation; n = 6. For all panels, ±SD is shown. n indicates biological replicates. P value was determined by two-tailed t test unless stated otherwise. Source data are provided as a Source Data file.