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. 2021 Jan 14;10:568433. doi: 10.3389/fonc.2020.568433

Figure 1.

Figure 1

The different bronchoalveolar lavage fluid (BALF) glycopatterns in benign pulmonary diseases (BPD), adenocarcinomas (ADC), squamous carcinomas (SCC), and small cell lung cancer (SCLC) using a lectin microarray, respectively. (A) The layout of the lectin microarrays. Each lectin was spotted in triplicate per block with quadruplicate blocks on one slide. Cy3-labeled BSA was spotted as a location marker and unlabeled BSA as a negative control. (B) The binding profiles of Cy3-labeled BALF glycoproteins bound to the lectin microarrays. The lectin microarrays revealed significant signal differences among BPD, ADC, SCC, and SCLC marked with white frames. While the significant differences among three types LC marked with red frames. (C) Heat map and unsupervised average linkage HCA of the normalized data of 37 lectins in the 281 BALF samples. Each sample were listed in columns, and the lectins were listed in rows. The color and intensity of each square indicated expression levels relative to the other data in the row. Red, high; green, low; black, medium.