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. 2021 Jan 27;11:2322. doi: 10.1038/s41598-021-81939-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Masson’s trichrome stains of 10-week regenerated rat bladder tissue following augmentation (40 ×). (A) Images of female and male bladder tissue sections show the distribution of muscle and collagen in the regenerated tissue that is representative of the animals in that graft group (n = 4–6 before subsets; 10 images per animal). Analysis of the regenerated tissue examines muscle tissue area as a percent of total regenerated bladder tissue for each group further divided into (B) stone negative (S−) and (C) stone positive (S+) rats. Further analysis shows a potential trend towards increased percent muscle area at higher seeding densities. In S− F/POC and F/SIS groups, a greater fraction of animals in each seeding group achieved ≥ 45% muscle as seeding densities increased, however, group differences were generally not statistically significant. A comparison of seeding groups CD34-50/MSC15 and CD34-100/MSC15 (F/POC S−) generated the only statistically significant group difference (p < 0.05). Scale bar: 50 μm. Error bars represent ± SE.