Table 3.

ITC measurements of NAD metabolites bound to MERS-CoV macro domain.

Ligand bound at 298 K	n	Kd (μM)	ΔH (KJ/mol)	−TΔS (KJ/mol)	ΔG (KJ/mol)
ADPR	1.25 ± 0.08	3.67 ± 2.03	−64.21 ± 4.00	32.85 ± 2.96	−31.37 ± 1.69
NAD	0.938 ± 0.13	>1000	1.18 ± 1.09	−15.9 ± 1.09	−17.12 ± 0.00
ATP	1.28 ± 0.23	289.30 ± 32.28	−0.431 ± 0.26	−19.77 ± 0.48	−20.20 ± 0.28
ADP	1.08 ± 0.06	70.29 ± 29.00	−16.11 ± 7.54	−7.77 ± 6.32	−23.88 ± 1.23
AMP	1.05 ± 0.38	191.23 ± 67.29	−11.19 ± 3.02	−10.12 ± 2.39	−21.31 ± 0.81
Ligand bound at 308 K	n	Kd (μM)	ΔH (KJ/mol)	−TΔS (KJ/mol)	ΔG (KJ/mol)
ADPR	1.20 ± 0.19	26.92 ± 13.47	−18.05 ± 18.45	−9.30 ± 17.61	−27.36 ± 1.33
NAD (WT)	1.04 ± 0.12	54.34 ± 2.81	−2.73 ± 3.66	−22.59 ± 3.77	−25.32 ± 0.13
NAD (G45V)	1.31 ± 0.10	154.50 ± 42.19	−76.05 ± 41.49	53.35 ± 41.50	−22.70 ± 0.71
NAD (I129F)	ND
ATP	ND
ADP	ND
AMP	ND

ND not determinable.