Fig. 5. Inducible ANKS1A ablation results in a rotational polarity defect, affecting the SDAs.

a Control and iKO mice were generated via five separate daily TM injections from P30 to P35. b The mice were sacrificed at P42 for live LW preparation, and the experiments were performed essentially as described in Fig. 3. c Thirty-five consecutive frames of Supplementary Movie 3 were merged into a single picture. Scale bar for b, c, 200 μm. d Data presented in b were used to calculate an average bead speed based on three independent experiments. Data represent mean ± SD. e Double staining for both ODF2 (green) and CNTRL (red) permitted the visualization of rotational polarity. f, g Experiments were performed as described in Fig. 4b, c. h, i Experiments were performed as described in Fig. 2e, f. Scale bar for f, h, 1 μm. Control set, n = 99 cells; ANKS1A iKO, n = 71 cells.