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. 2021 Jan 27;7:9. doi: 10.1038/s41522-020-00177-1

Fig. 4. Disruption of terminal reductase activity abrogates the biofilm response.

Fig. 4

a Schematic of the DMSO reductase encoding dmsEFABGH operon of Shewanella algae. b DMSO reduction of the WT strain and dmsB in-frame deletion mutant (****P < 0.0001, two-tailed unpaired t test) as evidenced by measuring DMS production of three independent cultures supplemented with 35 mM DMSO. c Biofilm formation of S. algae CECT 5071 WT and ∆dmsB in the absence or presence of DMSO (****P < 0.0001, two-tailed unpaired t test). Data represent the average and SD of three biological replicates with seven technical replicates each. d Schematic of the periplasmic nitrate reductase NAP-α encoding operon napEDABC and NAP-β encoding operon napDAGHB of S. algae. e Nitrate reduction by the single mutants ∆napABC (α−) and ∆napA (β−), and the double mutant ∆napABC ∆napA (αβ−) as determined by nitrite accumulation with respect to the WT strain upon supplementation with 35 mM nitrate (****P < 0.0001; one-way ANOVA followed by Dunnett’s post-hoc test; ND no nitrite production detected). Data represent the average and SD of three biological replicates with four technical replicates per sample. f Biofilm formation of S. algae CECT 5071 WT and nitrate reduction mutants in the absence or presence of sodium nitrate (****P < 0.0001, one-way ANOVA followed by Dunnett’s post-hoc test).