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. 2021 Jan 27;12:610. doi: 10.1038/s41467-020-20586-2

Fig. 2. Endophilin A2 deficiency protects against autoimmunity in rodents.

Fig. 2

a Schematic picture of the SH3gl1 gene with the location of the inserted ERVI long-term repeat element and location of the primer pairs used for quantification of the H3K4me3 and H4Ac levels. b Fold change of H3K4me3 levels in 3 DA and 3 DAMut naïve aged matched rats before (Primer pair 1 and 2) and after (Primer pair 3) the insertion in the SH3gl1 gene. c Fold change of H4Ac levels in 3 DA and 3 DAMut naïve aged matched rats before (Primer pair 1 and 2) and after (Primer pair 3) the insertion in the SH3gl1 gene. d Fold change of the SH3gl1 gene expression in PBMCs from 5 DA and 6 DAMut naïve aged matched rats. Relative fold change calculated to one DAMut reference sample. e Western blot analysis of the expression of the Endophilin A2 protein in brain samples of three DA and DAMut rats. Histone 2B was used as loading control. f Mean arthritis score after collagen-induced arthritis in 9 SH3gl1 knockouts and 16 wild-type littermates. Arthritis data has been reproduced twice with the same results. g Mean EAE score after spinal cord homogenate induced EAE in 10 DA and 10 DAMut rats. h Incidence of collagen-antibody induced arthritis in 10 SH3gl1−/− and 10 SH3gl1+/+ wild-type littermates. i Mean arthritis score of sick mice from above experiment after collagen-antibody induced arthritis in 4 SH3gl1 knockouts and three wild-type littermates. Non-parametrical Mann–Whitney U test was used for statistical evaluation of data. Data are presented as mean with error bars indicating ±SEM with each dot representing an individual value.