Fig. 3. T cells regulate Endophilin A2 mediated protection against arthritis.

a Expression of SH3gl1 in sorted T cells from lymph nodes from DA rats at Day 0 and Day 8 after pristane immunization. b Mean arthritis score after adoptive transfer of pristane-primed DA and DA^Mut cells to 9 respectively 4 naïve DA recipients. The adoptive transfer experiment has been reproduced a total of three times with the same results. c Mean arthritis score after GPI-induced arthritis in 17 and 19 TCRβ knockout recipients that had received either SH3gl1^−/− or SH3gl1^+/+ thymocytes respectively, 7 days prior to disease induction. Data is pooled from three individual experiments. d Number of antigen specific IL-2, IL-17A and IFNg producing T cells after recall stimulation with GPI peptide and IL-2 producing cells after ConA stimulation day 44 after GPI-induced arthritis. Data from 9 TCRβ knockout mice reconstituted with wild-type T cells and 8 TCRβ knockout mice reconstituted SH3gl1 T cells. Recall experiment has been repeated twice in SH3gl1^−/− mice. e Frequency of double negative (DN), double positive (DP), single positive CD4 (SP CD4), and single positive CD8 (SP CD8) thymocytes in 8 SH3gl1^−/− and 8 SH3gl1^+/+ mice. Data has been reproduced twice. f Frequency of CD25, CD44, PD-1, CD73⁺FR4⁺, Ki-67, and FoxP3 positive cells in CD4⁺TCRb⁺ cells from inguinal lymph nodes ten days after GPI-peptide immunization from 6 SH3gl1^−/− and 6 SH3gl1^+/+ mice. g Mean arthritis score in naïve DA recipients after adoptive transfer of pristane-primed DA^Mut cells only, DA cells only or DA cells in addition with DA^Mut cells, 7 rats per group. h Arthritis sum score of the DA recipients above after adoptive transfer of pristane-primed DA cells only or addition with DA^Mut cells, 7 rats per group. Non-parametrical Mann–Whitney U test was used for statistical evaluation of data. Data are presented as mean with error bars indicating ±SEM with each dot representing an individual value.