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. 2021 Jan 27;12:610. doi: 10.1038/s41467-020-20586-2

Fig. 5. EA2 has a conserved function in human T cells and is upregulated in RA patients.

Fig. 5

a TCR internalization in SH3GL1 CRISPR knockout Jurkat cells (T3-5 and T3-21) and wild-type control (T3-23). b Representative western blot analysis from two experiments of phosphorylated ZAP70 and ERK1/2 in T cells from in SH3GL1 CRISPR knockout (T3-5 and T3-21), wild-type control (T3-23), and Jurkat (J) cells with anti-CD3/CD28 for 0, 3, and 7 min. Histone 2B was used as loading control. c Correlation of CD3e and SH3GL1 gene expression in whole blood of healthy controls and RA patients. Each circle represents one individual. d Normalized SH3GL1 gene expression to CD3e gene expression in whole blood of 35 healthy controls and 36 RA patients, randomly selected from the cohort used in c. Each circle represents one individual. e SH3GL1 gene expression in sorted CD4+ T cells from 10 healthy controls and 8 RA patients (data accessible at NCBI GEO database, accession GSE4588). Non-parametrical Mann–Whitney U test was used for statistical evaluation of data. Data are presented as mean with error bars indicating ±SEM with each dot representing an individual value.