Figure 4.

The silencing of ATP4B in human hepatocellular carcinoma (HCC) and pancreatic cancer cell lines not due to epigenetic regulation. (A, B) Semiquantitative RT-PCR of the mRNA level of ATP4B expression in human HCC cell lines and immortalized liver embryonic cell line L02 (A) or in human pancreatic cancer cell lines (B). HCC, hepatocellular carcinoma. (C, D) MSP analysis of ATP4B methylation in HCC cell lines (C) or pancreatic cell lines (D). (E) RT-PCR of the expression of ATP4B and MT2A in human BGC823 cell line treated with DATS (40 μM, 12 h) as described. MT2A was used as a positive control for DATS treatment. MT2A, metallothionein 2A.