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. 2021 Jan 27;11:2324. doi: 10.1038/s41598-021-81752-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cross-reactivity of antisera to CCHFV and NSDV NPs. ELISA plates were coated with 10 µg/ml of purified CCHFV NP, NSDV NP, or the mock antigen. Serial dilutions (1:100, 1:1,000, 1:10,000, and 1:100,000) of mouse antiserum to CCHFV, to NSDV NP, and naïve mouse serum were used as primary antibodies and incubated for 2 h at 4 °C. Peroxidase conjugate goat anti-mouse IgG (H + L) was used for detection of bound antibodies. To offset the nonspecific antibody reaction, the OD value of the mock antigen was subtracted for each dilution.