Figure 2.
Overexpression of IRF7 inhibited prostate cancer bone metastasis. (A) The expression of IRF7 mRNA was determined by qRT-PCR. The expression of IRF7 mRNA in PC-3/IRF7 and LnCap/IRF7 cells was increased compared with the control cells. (B) The expression of IRF7 protein was determined by Western blot. The expression of IRF7 at the protein level in PC-3/IRF7 and LnCap/IRF7 cells was increased compared with the control cells. (C) PC-3/IRF7 and PC-3/NC cells (2 × 106 per mouse) were injected into the caudal vein of male nude mice. At 30 days after injection, mice were anesthetized, and digital radiographs were taken. The red arrows in the X-ray images show the metastatic bone lesions of PC-3/IRF7 and PC-3/NC cells after injection. (D) The lesions were confirmed by staining sections with hematoxylin and eosin. (E) Quantitative real-time PCR detection against the GFP gene of metastatic tumor burden in bone was conducted. *p < 0.05. (F) The growth curves of the prostate tumors in xenograft nude mice were constructed.