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. 2017 Sep 21;25(8):1261–1267. doi: 10.3727/096504017X14871164924588

Figure 5.

Figure 5

Knockdown of FOXK1 prevented the activation of the Wnt/β-catenin pathway in prostate cancer cells. (A) PC-3 cells were transfected with shFOXK1 or shNC for 48 h, respectively. Thereafter, total protein lysates were analyzed by Western blot using the indicated antibodies. GAPDH served as loading control. (B) PC-3 cells were treated with different concentrations of the inhibitor of β-catenin pathway (XAV939) for 24 h, and cell viability was assessed. PC-3 cells were transfected with shFOXK1 or shNC in the presence or absence of XAV939 (50 nM) for 24 h. (C) Transwell migration assay was used to examine cell migration. (D) Cell invasion was assessed by the Matrigel invasion assay. Data are representative of three independent experiments performed under identical experimental conditions. *p < 0.05.