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. 2017 Aug 7;25(7):1189–1197. doi: 10.3727/096504017X14865126670075

Figure 3.

Figure 3

LINC0086 interacts with miR-214 and regulates its expression. (A) qPCR was performed to detect the expression of miR-214 in C666-1 and HK-1 cells after LINC0086 transfection. (B) The wild type (WT) and mutant type (MT) of LINC0086 3′-UTR, and the binding sites were shown. (C) The relative luciferase activities were inhibited in the C666-1 and HK-1 cells cotransfected with wild-type LINC0086 3′-UTR and miR-214, and not with the mutant type. Firefly luciferase activity was normalized to Renilla luciferase. (D) Association of LINC0086 and miR-214 with Ago2 in C666-1 and HK-1 cells. Cellular lysates from C666-1 and HK-1 cells were used for RNA immunoprecipitation (RIP) with antibody against Ago2. LINC0086 and miR-214 expression levels were detected using qRT-PCR. *p < 0.05, **p < 0.01, ***p < 0.001 versus NC group.