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. 2017 Mar 13;25(3):373–380. doi: 10.3727/096504016X14741486659473

Figure 2.

Figure 2

Overexpression of CPS1-IT1 inhibited colony formation and cell proliferation in lung cancer cell lines. (A) An expressing plasmid for lncRNA CPS1-IT1 was constructed into pcDNA3.1 vector and transfected into A549 and 95D cells, after which RT-PCR analysis revealed that the relative transcript levels of CPS1-IT1 were upregulated in both cell lines. (B) Colony formation assays were performed in A549 and 95D cells upon CPS1-IT1 plasmid transfection. (C) A549 cells were transfected with or without CPS1-IT1-expressing plasmid and subjected to cell viability assays for a consecutive 5 days. (D) 95D cells were transfected with plasmids in the presence or absence of CPS1-IT1 overexpression and subjected to cell viability assays. Cell proliferative rate was detected each day for a consecutive 5 days. *p < 0.05 versus Control group in A549 cells. #p < 0.05 versus Control group in 95D cells.