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. 2017 Nov 2;25(9):1529–1541. doi: 10.3727/096504017X14888987683152

Figure 4.

Figure 4

microRNA (miR)-135a is a target of UCA1. (A) The targeting relationship between miR-135a and UCA1 was predicted through bioinformatics. (B) Relative expression of miR-135a in PC tissues and normal tissues was detected through qPCR (**p < 0.01). (C) Relative expression of miR-135a in human PC cell lines (SW1990, BxPC-3, MiaPaCa-2, PANC-1, and CAPAN-1) and HPDE cells were detected through qRT-PCR (**p < 0.001 vs. HPDE group). (D) Expression of miR-135a in PC tissues and normal tissues was detected by Western blotting. (E) Expression of miR-135a in related cell lines was detected by Western blotting. β-Actin was used as an endogenous reference. (F) The expression of miR-135A was measured by Western blotting in SW1990 cells transfected with long noncoding RNA (lncRNA) UCA1 and/or miR-135a mimic or mock. (G) The expression of miR-135a was measured through Western blotting in SW1990 cells transfected with UCA1 siRNA and/or miR-135a inhibitor or mock, respectively. (H) The wild-type (Lnc-UCA1-WT) or mutant (Lnc-UCA1-MUT) luciferase reporter was cotransfected into SW1990 cells with miR-NC or miR-135a mimic. The luciferase reporter assay was conducted to detect the luciferase activity in SW1990 cells (**p < 0.001 vs. Lnc-UCA1-WT group).