Figure 2.

Ectopic expression of PRSS8 inhibited tumor growth in vitro and in vivo. A549 cells were transfected with PRSS8 or mock for 24 h. (A) The corresponding transfection efficiency was detected by Western blot and qRT-PCR. (B) Cell proliferation was evaluated using the WST-1 assay. PRSS8 cells (5 × 10⁶) and control cells were injected subcutaneously into the left and right dorsal flanks of 4-week-old female BALB/c-nu mice. (C) Tumor volume was monitored every 5 days. (D) After 20 days, the animals were sacrificed, and the tumors were excised and weighed. Experiments were performed in triplicate. *p < 0.05 versus mock group.