Figure 4.

Effect of chalcone derivatives on the production of nitric oxide by L. infantum-infected macrophages. L. infantum-infected macrophages were treated with increasing concentrations of (A–D) chalcone derivatives (11–54 μM) or (E) the reference drug fungizone (0.13–0.54 μM) and nitrite concentration was measured by the Griess reaction. Control represents the amount of nitric oxide produced by L. infantum-infected macrophages in the absence of any treatment. Statistical analysis of the differences between mean values obtained for the experimental groups was done by ANOVA with Tukey's post hoc test. P-value < 0.0005 (three asterisks), p < 0.0001 (four asterisks) were considered significantly different from the control.