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Knockdown of TMEM45B inhibited osteosarcoma cell proliferation in vitro. U2OS cells were transfected with shTMEM45B or shNC for 48 h, respectively. (A) The expression of TMEM45B at both the mRNA and protein levels was analyzed by qRT-PCR and Western blotting. (B) The CCK-8 assay was performed to determine the effect of shTMEM45B on cell proliferation. *p < 0.05, compared with the shNC group.
