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. 2021 Jan 14;8:575903. doi: 10.3389/fcell.2020.575903

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Copyright © 2021 Min, Zhang, Wang, Qi, Song, Bibi, Zhang, Ma, Zhao, Yu and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MICAL-L2 overexpression enhanced the proliferative ability of human non-small cell lung carcinoma (NSCLC) cells. (A,B) Total protein extracts from PC9 cells transfected with MICAL-L2 expression plasmids for 48 h were assessed for c-Myc expression. Data are presented as means ± SD of 3 determinations. ***P < 0.001 relative to control cells. (C) EdU staining in MICAL-L2-overexpressing PC9 cells. Data are presented as means ± SD of 5 independent determinations. (D) The viability of PC9 cells transfected with empty vector or MICAL-L2 expression plasmids was measured by CCK-8 assay. Data are presented as means ± SD of 10 determinations. (E) Representative immunofluorescence images of c-Myc staining in PC9 cells transfected with MICAL-L2 expression plasmids. Scale bar, 5 μm.**P < 0.01, ***P < 0.001 relative to control cells.