Skip to main content
. 2021 Jan 27;12(1):131. doi: 10.1038/s41419-021-03401-8

Fig. 2. Cdk5rap3 deletion impairs fate specification of Paneth cell lineage.

Fig. 2

a Quantitative RT-PCR analysis of specific genes involved in intestinal stem cell renewal and differentiation. Total RNA was isolated from intestinal scrapes and used for qRT-PCR analysis. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n = 4 mice per genotype). b Proliferating cells at the crypt base of wild-type and Cdk5rap3 deficient small intestine. Ileal sections were so-stained with PCNA and Cdk5rap3 antibodies. c Olfm4 staining of wild-type and Cdk5rap3∆/∆IEC ileal sections. Olfm4+ cells per crypt were scored and compared. **p < 0.01. (n = 4 mice per genotype). d TUNEL staining of wild-type and Cdk5rap3∆/∆IEC ileal sections. TUNEL positive cells per crypt were scored from four mice of each genotype.