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. 2021 Jan 27;11:2315. doi: 10.1038/s41598-021-81898-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histograms of the distribution of head and tail protrusion lengths. (a) Top panel: schematic representation of an FA in the two colour SIM images clarifying the method. Bottom panels: pax/pax reference histograms showing the distribution of the apparent head (top) and tail (bottom) protrusion lengths measured in the SIM images of the pax/pax reference set of U2OS cells co-expressing paxillin-mCherry and paxillin-GFP. These histograms are further systematically shown in grey in each presented histogram in all figures. (b–f) Histograms of FA head and tail protrusion lengths for the indicated protein pairs co-expressed in U2OS cells. Red protrusion lengths are plotted from right to left (i.e. negative green protrusion lengths) and green from left to right. The p values are generated by comparison of the experimental histogram (black line) and the reference histogram (grey) in two-sided Mann Whitney U tests, n represents the number of analysed FAs. Vertical lines: significant median protrusion lengths. Number of imaged cells from b to f: 15, 52, 17, 18 and 23. The pax/pax reference histograms are shown in grey.