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Wnt induced β-catenin accumulation in the nucleus. (A) Wnt induced β-catenin accumulation in the nucleus dose dependently. (B) β-Catenin expression in whole-cell lysate. Immunoblot was done to see nuclear translocation. CREB was used as a loading control of nuclear protein. (C) Activity of GSK-3β was not changed by Wnt agonist. Phosphorylation of GSK-3β was determined by Immunoblot.
