Figure 4.

Wnt agonist induced ER stress- and caspase-independent cell death. (A) Phosphorylation of PERK and eIF2α, and expression of CHOP were determined by immunoblot. DMSO alone was used as control. Cells were incubated with 10 μM of Wnt agonist for 1 h for identifying PERK/eIF2α and 24 h for CHOP. (B) Caspase (Z-VAD-FMK) or caspase 3 inhibitors (DEVD-FMK and IETD-FMK) did not influence Wnt-induced loss of cell viability. Cell viability was determined by MTT assay. *p < 0.05, significantly different from control.