Knockdown of TRIM59 inhibits cell migration and invasion abilities in cervical cancer. (A, B) Cell migration (A) and invasion abilities (B) were assessed using Transwell assay. After each treatment, cells were allowed to migrate for 8 h before proceeding to crystal violet staining. (C, D) After staining, three randomly selected fields were photographed under microscopy. The cell numbers in the three images were then averaged and shown in bars. Cell migration (C) and invasion (D) were shown separately in the histograms. (E) Wound-healing assay, which reflects cell migration ability, was conducted. The percentage of wound closure in HeLa and SiHa cells was calculated 24 h after the wound scratch.