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. 2017 Mar 13;25(3):445–454. doi: 10.3727/096504016X14747368729786

Figure 3.

Figure 3

REST regulates MMP9 expression by repressing its promoter activity. (A) Induced REST in MDA-MB-231 cells reduced MMP9 mRNA levels. (B) Induced REST in MDA-MB-231 cells reduced MMP9 protein levels. (C) Luciferase reporter gene assay using the reporter vector containing the promoter of MMP9 gene. MDA-MB-231 cells were cotransfected with MMP9 promoter together with different amounts of a REST expression lentivirus. REST reduces luciferase expression in a concentration-dependent manner in MDA-MB-231 cells. Data are expressed as mean ± standard deviation from three independent experiments. **p < 0.01, ***p < 0.001 versus negative control. Con, control; NC, negative control.