Figure 3.

Effect of GAS5 overexpression on tumorigenesis and radiosensitivity of NSCLC cells. A549 and H1975 cells were transfected with pcDNA-GAS5 or empty vector and cultured for 48 h. (A) The expression of GAS5 in transfected A549 and H1975 cells was examined by qRT-PCR. (B) Cell viability at 24, 48, and 72 h in transfected A549 and H1975 cells was detected by MTT assay. (C) Transwell invasion assay was performed to determine cell invasiveness of transfected A549 and H1975 cells. (D) The clonogenic survival curves of transfected A549 and H1975 cells were established by the colony formation assay. *p < 0.05.