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The interaction between GAS5 and miR-135b. (A) The predicted wild-type binding sites for miR-135b in GAS5 sequences and its mutant. (B) Luciferase reporter assay was performed to measure luciferase activities in A549 and H1975 cells cotransfected with pGL3-GAS5-wt or pGL3-GAS5-mut and miR-135b or miR-control. (C) qRT-PCR analyses of miR-135b expression in A549 and H1975 cells transfected with si-GAS5, pcDNA-GAS5, or respective controls. *p < 0.05.
