Skip to main content
. 2017 Apr 14;25(4):605–615. doi: 10.3727/096504016X14767450526417

Figure 3.

Figure 3

CISD2 silencing inhibited the survival and growth of pancreatic cancer cells. (A) We applied lentivirus carrying shRNA specifically targeting CISD2 (shCISD2) to downregulate the level of CISD2 in PANC-1 and SW1990 cells. The efficiency of knocking down CISD2 was determined by Western blot. Scr, scramble. (B) Relative level of CISD2 in (A) was measured using ImageJ and normalized to GAPDH. (C, D) The effect of CISD2 defect on the growth rate of PANC-1 and SW1990 cells was tested with the MTT assay. (E) The TUNEL assay indicated that the CISD2 defect increased the percentage of apoptotic cells in PANC-1 cells. (F) Flow cytometry confirmed the increase in number of necrotic and apoptotic cells by shCISD2 in SW1990 cells. Lower left (LL) quadrants: the live cells. Lower right (LR) quadrants: the early apoptotic cells. Upper right (UR) quadrants: terminal apoptotic cells. Upper left (UL) quadrants: the necrotic cells. Data were presented as mean ± SD from at least three independent experiments. *p < 0.05 and **p < 0.01 compared with control group and ##p < 0.01 compared with the indicated groups.